Bovine Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : A8YXZ8
Abbreviation : TOMM5
Alternative Names : RP11-613M10.3; C9orf105; RP11-263I4.1; Tom5; bA613M10.3; mitochondrial outer membrane protein TOM5|translocase of outer mitochondrial membrane 5 homolog
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?6.3%
Inter-AssayCV : ?8.9%
Recovery : 1
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TOMM5 in samples. An antibody specific for TOMM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TOMM5 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The fungal preprotein translocase of the mitochondrial outer membrane (TOM complex) comprises import receptors Tom70, Tom20, and Tom22, import channel Tom40, and small Tom proteins Tom5, Tom6, and Tom7, which reg µLate TOM complex assembly. These components are conserved in mammals; unlike the other components, however, Tom5 and Tom6 remain unidentified in mammals. These small Tom proteins are associated with Tom40 in the TOM complex. Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex. Conversely, knockdown of hTom40 decreased the level of all small Tom proteins. Matrix import of preprotein was affected by double knockdown of any combination of small Tom proteins. These res µLts indicate that human small Tom proteins maintain the structural integrity of the TOM complex.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).