Bovine Transportin 1 (TNPO1) ELISA Kit
Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q3SYU7
Abbreviation : TNPO1
Alternative Names : IPO2; KPNB2; MIP; MIP1; TRN; M9 region interaction protein|importin 2|importin beta 2|karyopherin (importin) beta 2
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?7.7%
Recovery : 0.89
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TNPO1 in samples. An antibody specific for TNPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TNPO1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Targeting of most nuclear proteins to the cell nucleus is initiated by interaction between the protein's nuclear localization signal (NLS) and the importin, or karyopherin, receptor complex. An importin heterodimer recognizes the NLS protein in the cytoplasm via its alpha subunit and, via its beta subunit, docks the complex to a subset of peptide repeat-containing proteins known as nucleoporins. Transportin-1 is the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).