Bovine Trimethyllysine dioxygenase mitochondrial (TMLHE) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q0VC74
Abbreviation : TMLHE
Alternative Names : BBOX2; FLJ10727; TMLH; XAP130; butyrobetaine (gamma); 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2|epsilon-trimethyllysine 2-oxoglutarate|epsilon-trimethyllysine hydroxylase
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?7.8%
Recovery : 1.02
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TMLHE in samples. An antibody specific for TMLHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMLHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TMLHE is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMLHE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Epsilon-N-trimethyllysine hydroxylase (EC 1.14.11.8) catalyzes the conversion of epsilon-N-trimethyllysine to beta-hydroxy-N-epsilon-trimethyllysine in the first step of L-carnitine biosynthesis. The deduced 421-amino acid human TMLHE and 405-amino acid rat Tmlhe proteins have calc µLated molec µLar masses of 49.5 kD and 47.5 kD, respectively, and share 92% amino acid identity. Subcell µLar fractionation localized rat Tmlhe to mitochondria. Rat Tmlhe was syntheVolumed as a 47.5-kD precursor that is processed to an approximately 43-kD protein, most likely thro µgh cleavage of a mitochondrial targeting signal. Gel filtration analysis showed rat Tmlhe as an 87-kD band, s µggesting that Tmlhe forms a homodimer.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).