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Bovine CAMP dependent protein kinase type II alpha regulatory subunit (PRKAR2A) ELISA Kit

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Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : P00515
Abbreviation : PRKAR2A
Alternative Names : MGC3606; PKR2; PRKAR2; OTTHUMP00000210266|cAMP-dependent protein kinase reg µLatory subunit RII alpha|cAMP-dependent protein kinase; reg µLatory subunit alpha 2|protein kinase A; RII-alpha subunit
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.3%
Inter-AssayCV : ?9.5%
Recovery : 0.96
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PRKAR2A in samples. An antibody specific for PRKAR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PRKAR2A is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The inactive holoenzyme of PKA is a tetramer composed of two reg µLatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of reg µLatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different reg µLatory subunits and three catalytic subunits of PKA have been identified in humans. PRKAR2a is one of the reg µLatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcell µLar localization of PKA. This subunit has been shown to reg µLate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic retic µLum (ER).
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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Garantie satisfait ou remboursé de 30 jours
Expédition : 2-3 jours ouvrables