Cleaved PARP (Asp214) Antibody

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : PARP1
GeneID : 142
Uniprot : P09874
Specificity : Cleaved-PARP (Asp214) Antibody detects endogenous levels of fragment of activated PARP res µLting from cleavage adjacent to Asp214.
Immunogen : The antiserum was produced against syntheVolumed peptide derived from human PARP.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 14 months from date of receipt.
Reactivity : Human,Mouse,Rat
Prediction : Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
Application : WB,ELISA(peptide)
Dilution : WB 1 : 500-1 : 2000, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2835327
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF7023-BP
Des# Blocking Peptide : Cleaved-PARP (Asp214) Blocking peptide
Blocking Peptide Price : $549 CAD