Cyclin B2(X29.2) , CF647 conjugate , 0.1mg / mL

In eukaryotic cells , mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor , M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2) , a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control , of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme , and tyrosine dephosphorylation , occurring at the onset of mitosis , directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase , but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase , of which Cdc2 is the physiological substrate. The two B type cyclins , cyclin B1 and cyclin B2 , have been shown to have distinct tissue distributions._x000D_ _x000D_ Primary antibodies are available purified , or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets , because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_