BRD4 Antibody FITC conjugated

Volume : 50 µg
Clone Number :
Aliases : Brd4 antibody; BRD4-NUT FUSION antibody; BRD4-NUT fusion oncoprotein antibody; BRD4_HUMAN antibody; Bromodomain containing 4 antibody; bromodomain containing protein 4 antibody; Bromodomain-containing protein 4 antibody; CAP antibody; chromosome associated protein antibody; HUNK1 antibody; HUNKI antibody; MCAP antibody; Mitotic chromosome-associated protein antibody; Protein HUNK1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : O60885
Immunogen : Recombinant Human Bromodomain-containing protein 4 protein (150-200AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications :
Background : Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription reg µLation. Remains associated with acetylated chromatin thro µghout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in reg µLating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters : BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative reg µLators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of \'Ser-2\' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of \'Ser-2\' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed : 22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a reg µLator of p53/TP53-mediated transcription : following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : FITC
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : BRD4
Research Areas : Epigenetics and Nuclear Signaling; Cancer